FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (24 ): 38-41.doi: 10.7506/spkx1002-6630-200924005

Previous Articles     Next Articles

Protein Extraction from Rice Endosperm Using Reductant-alkali Method

LIU Chun-you,LIU Yong-le*,YU Jian,YI Cui-ping,LI Xiang-hong   

  1. College of Chemistry and Biology Engineering, Changsha University of Science and Technology,
    Changsha 410004, China
  • Received:2009-07-01 Online:2009-12-15 Published:2010-12-29
  • Contact: LIU Yong-le*, E-mail:lyle19@163.com

Abstract:

In this study, a combinatorial method of reductant-alkali solution and acid precipitation was used to extract protein from rice endosperm. The optimal condition for rice protein extraction was determined through single factor and orthogonal experiments to be 0.05 mol/L sodium hydroxide, 0.50% sodium sulfite, 2.50% dithiothreitol, 1:12 (g/ml) of solid-liquid ratio, and 4 h of extraction time. Extraction rate of rice protein was 95.26% under this optimal condition.

Key words: rice protein, reductant, alkali solution, extraction

CLC Number: