Abstract:

Supercritical CO2 fluid chromatography (SFC) was used to separate isomers, eugenol and isoeugenol in this paper. Effects of modifier, column pressure and column temperature on separation efficiency was evaluated by resolution factor under optimal silica column or cyano column. Results indicated cyano column provided better separation efficiency due to successful separation of trans- and cis-isomers of isoeugenol. The optimal separation condition for eugenol and isoeugenol was cyano column (250 mm × 4.6 mm, 5μm), UV detection wavelength at 283 nm, 8% modifier in methanol as mobile phase, 1.5 ml/min of flow rate for mobile phase, 40 ℃ of column temperature, 8 MPa column pressure, 10 μl of sample loading amount, and 43.7 mg/ml sample. The resolution factor was 2.41 with 7 min of running time under this optimal separation condition.

Key words: supercritical CO2 fluid chromatography, eugenol, isoeugenol, separation