Abstract:

In this study, binding capacity between sodium dodecyl sulfate (SDS) and protein was used to evaluate protein hydrophobicity. SDS and protein were mixed in a buffer with certain concentration and pH. Followed by dialysis of the SDSprotein mixture, the bound SDS was determined through Epton s method. The optimal determination condition was achieved to be 24 h of dialysis, 30 ℃ of dialysis temperature, 40 ml of SDS solution and pH 8. Compared with fluorescence probe method, the SDS binding method exhibited a good correlation with it.

Key words: protein, hydrophobicity, SDS-binding method, dialysis