Factors Affecting the Determination of Superoxide Dismutase Activity

doi:10.7506/spkx1002-6630-201009048

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (9): 216-218.doi: 10.7506/spkx1002-6630-201009048

• Bioengineering • Previous Articles Next Articles

Factors Affecting the Determination of Superoxide Dismutase Activity

ZHAO Jian1，LI Xiang2，LU Zheng1，WEN Jing1,*

1. Beijing Key Laboratory of Bioactive Substances and Functional Foods, Beijing Union University, Beijing 100191, China；
2. Beijing Institute for Drug Control , Beijing 100035, China

Received:2009-07-16 Online:2010-05-01 Published:2010-12-29
Contact: WEN Jing E-mail:wenjing@ygi.edu.cn

Abstract

Abstract:

Objective: To verify the factors for affecting the determination of superoxide disutase (SOD) activity in xanthine oxidase method and the evaluation error for antioxidant functions of healthy food. Methods: Effects of the addition of albumin, total protein, triglyceride, glucose, vitamin C and glutathione on SOD determination in xanthine oxidase method and on specific activity of SOD in mice treated with vitamin C through gastric perfusion. Results: In xanthine oxidase method, the specific activity of SOD still could be detected although SOD was inactivated. The addition of above six substances could disturb determination results of SOD activity. However, dialysis could eliminate the interference from small molecular substances in serum. Conclusion: Xanthine oxidase method for detecting SOD activity is a non-specific method. Protein, fat, vitamin C and glutathione in serum can affect SOD activity to result in a positive error. Dialysis is a simple and effective method to remove these interference substances.

Key words: antioxidant healthy food, functional evaluation, superoxide dismutase, detection method

CLC Number:

TS207.3

ZHAO Jian1，LI Xiang2，LU Zheng1，WEN Jing1,*. Factors Affecting the Determination of Superoxide Dismutase Activity[J]. FOOD SCIENCE, 2010, 31(9): 216-218.

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Factors Affecting the Determination of Superoxide Dismutase Activity

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