Protective Effect of Opuntia milpa alta Polysaccharides on SDS-induced Damage of Human Cell Membrane and Mouse Hepatocyte DNA

doi:10.7506/spkx1002-6630-201011048

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (11): 226-230.doi: 10.7506/spkx1002-6630-201011048

• Nutrition & Hygiene • Previous Articles Next Articles

Protective Effect of Opuntia milpa alta Polysaccharides on SDS-induced Damage of Human Cell Membrane and Mouse Hepatocyte DNA

WANG Hai-tao1，HE Cong-fen1，DONG Yin-mao1，ZHANG Hui-ming1,2，PI Nan1，LIU Wen-ting1，ZHAO Hua1,*

1. Beijing Key Laboratory of Plant Resources Research and Development, Beijing Technology and Business University, Beijing
100048, China；2. Pharmaceutical College, Heilongjiang University of Chinese Medicine, Harbin 150040, China

Received:2009-10-14 Online:2010-06-01 Published:2010-12-29
Contact: ZHAO Hua E-mail:congfenhe@126.com

Abstract

Abstract:

Objective: To investigate the protective effect of Opuntia milpa alta polysaccharides (OMAP) on human red blood cell (RBC) membrane and mouse hepatocyte DNA damage induced by sodium dodecyl sulfate (SDS). Methods: RBC hemolysis test and Comet assay were used to determine the effect of OMAP on cell membrane damage and DNA damage, respectively. Three groups were designed for the experiments. The positive control group of cell system was given 40μg/mL SDS; OMAP control groups of cell system were cultured in media containing OMAP with varying concentrations (20, 40, 60, 80 μg/mL and 100μg/mL); OMAP + SDS groups of cell system were first treated with OMAP at varying concentrations prior to incubation in media with 40 μg/mL SDS. Results: OMAP presence at all concentration conditions could markedly prevent cell membrane and DNA damage induced by SDS, and an obvious dosage-response relationship was observed. Conclusion: OMAP has good potential to protect SDS-induced cell membrane and DNA damage.

Key words: Opuntia milpa alta polysaccharides, red blood cell hemolysis test, comet assay, cell membrane damage, DNA damage

CLC Number:

Q819

WANG Hai-tao1，HE Cong-fen1，DONG Yin-mao1，ZHANG Hui-ming1,2，PI Nan1，LIU Wen-ting1，ZHAO Hua1,*. Protective Effect of Opuntia milpa alta Polysaccharides on SDS-induced Damage of Human Cell Membrane and Mouse Hepatocyte DNA[J]. FOOD SCIENCE, 2010, 31(11): 226-230.

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Protective Effect of Opuntia milpa alta Polysaccharides on SDS-induced Damage of Human Cell Membrane and Mouse Hepatocyte DNA

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