FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (12): 67-70.doi: 10.7506/spkx1002-6630-201012015

• Technology Application • Previous Articles     Next Articles

Extraction of Total Flavonoids from the Peel of Ponkan Mandarin (Citrus reticulata Blanco) by Optimized Cellulase Hydrolysis

OUYANG Hui,YU Ji,ZHANG Yong-kang   

  1. Hunan Provincial University Key Laboratory of Plant Resources Conservation and Utilization, Jishou University,
    Jishou 416000, China
  • Received:2009-11-05 Revised:2010-02-01 Online:2010-06-15 Published:2010-12-29
  • Contact: OUYANG Hui, E-mail:oyhmail@163.com

Abstract:

The purpose of the present study was to investigate the use of response surface methodology based on Box-Benhnken experimental design to optimize the cellulase hydrolysis of Ponkan mandarin peel for the extraction of total flavonoids. The optimum hydrolysis conditions for the extraction of total flavonoids were determined to be: enzyme concentration 120 U/mL, hydrolysis temperature 52 ℃, length of hydrolysis time 140 min and pH 4.35. Under such conditions, the extraction yield of total flavonoids reached up to 1.85%.

Key words: Ponkan mandarin peel, total flavonoids, cellulase, response surface methodology (RSM), extraction

CLC Number: