Purification, Identification and Characterization of Membrane-bound Alcohol Dehydrogenase from Gluconobacter oxydans DSM 2003

doi:10.7506/spkx1002-6630-201013038

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (13): 164-168.doi: 10.7506/spkx1002-6630-201013038

• Bioengineering • Previous Articles Next Articles

Purification, Identification and Characterization of Membrane-bound Alcohol Dehydrogenase from Gluconobacter oxydans DSM 2003

WEI Liu-jing1，LIN Jin-ping1，YANG Xue-peng2，WEI Dong-zhi1,*

1. Newworld Institute of Biotechnology, State Key Laboratory of Bioreactor Engineering, East China University of Science and
Technology, Shanghai 200237, China；2. College of Food and Biological Engineering, Zhengzhou University of Light Industry,
Zhengzhou 450002, China

Received:2009-12-28 Online:2010-07-01 Published:2010-12-29
Contact: WEI Dong-zhi E-mail:weiliujing@ecust.edu.cn

Abstract

Abstract:

The membrane fraction of Gluconobacter oxydans DSM 2003 was collected by ultra-centrifugation, and an enzyme with dehydrogenase activity to 1,2-propanediol was isolated from the membrane fraction and purified by CM-cellulose column chromatography. The purified enzyme was composed of two subunits with molecular weights of approximately 80 and 50 kD, respectively. Meanwhile, this enzyme was confirmed to be alcohol dehydrogenase (ADH) by mass spectrometry. The optimal reaction pH and temperature for this enzyme were 5.5－6.0 and 30 ℃, respectively. Although this enzyme exhibited a broad substrate spectrum including primary and secondary alcohols, alcohols containing 3 hydroxyl groups and more failed to be oxidized by it. The activity of this enzyme was inhibited by most of the selected metal ions, especially by Cu2+ and Fe3+.

Key words: Gluconobacter oxydans, alcohol dehydrogenase, purification, identification, characterization

CLC Number:

Q816

WEI Liu-jing1，LIN Jin-ping1，YANG Xue-peng2，WEI Dong-zhi1,*. Purification, Identification and Characterization of Membrane-bound Alcohol Dehydrogenase from Gluconobacter oxydans DSM 2003[J]. FOOD SCIENCE, 2010, 31(13): 164-168 https://doi.org/10.7506/spkx1002-6630-201013038

[1]	SUN Tingting, XU Haonan, ZHANG Fang, WU Wei, CUI Can, YUAN Xiaoyong, CUI Yu, CHE Yan, SHAO Junxian, CHEN Hao, YU Hao. Preparation and Characterization of Anemarrhena asphodeloides Bge. Polysaccharides and Their Effects on Brain Tissue and Gut Microbiota in Aging Mice [J]. FOOD SCIENCE, 2026, 47(9): 190-202.
[2]	XU Fangqing, CAO Yujin, WU Yiyuan, DONG Xiaotong, HU Jiamiao. Application of Steam Explosion Technology in Plant Polysaccharides: from Efficient Extraction to Functional Enhancement [J]. FOOD SCIENCE, 2026, 47(7): 406-414.
[3]	ZHANG Yanqing, LI Qian, QIAO Mingwu, SHEN Yue, LI Ning, HUANG Xianqing, LEI Fengmei, SONG Lianjun. Preparation, Structural Characterization and in Vitro Biological Activities of Steam Explosion Modified Pea Protein-Derived Peptide-Chromium(III) Chelate [J]. FOOD SCIENCE, 2026, 47(6): 58-68.
[4]	ZHANG Zhe, YUAN Zijing, ZHANG Qiwen, WU Lijun, ZHANG Fuxian. Molecular Characterization and Pathogenicity Analysis of MBLs-Producing Pathogenic Escherichia coli O157:H12 Strains from Dairy Cows [J]. FOOD SCIENCE, 2026, 47(6): 155-166.
[5]	SHI Wanrong, SHEN Shuyue, WANG Qin, LI Zhengpeng, LI Tingting. Origin Traceability and Cultivation Mode Identification of Phellinus linteus Based on Hyperspectral Imaging Combined with Deep Learning [J]. FOOD SCIENCE, 2026, 47(6): 342-350.
[6]	GAO Yunqi, ZHANG Yi, ZHOU Mengzhou, CHEN Yang, HU Shan, SUN Zhouliang, GUO Hongtao, PING Lei, ZHENG Mingming. Research Progress in the Biosynthesis, Purification and Nutritional Application of Nervonic Acid [J]. FOOD SCIENCE, 2026, 47(5): 13-25.
[7]	TU Ran, LI Yan. Research Progress in Taste Mechanism and Debittering Technologies of Bitter Peptides [J]. FOOD SCIENCE, 2026, 47(5): 393-403.
[8]	BU Tingting, ZHOU Yexi, KONG Xiao, HUANG Zhenyu, CAO Xuelan, XIANG Wen, YANG Kai, CAI Ming, YANG Huimin, SUN Peilong. Purification, Identification, and Taste Characterization of Umami Peptides from Cooking Broth of Moso Bamboo Shoots [J]. FOOD SCIENCE, 2026, 47(4): 49-57.
[9]	JI Hongfei, HAN Xiao, YANG Qiuning, LU Yihan, JIANG Yanjun, DONG Xiaodan, DING Wenjie, JI Haiyu. Immunomodulatory Activity of Xylan Palmitate from Suillus luteus through Lactobacillus Enrichment in the Gut [J]. FOOD SCIENCE, 2026, 47(4): 139-150.
[10]	WANG Yanru, WANG Xinyue, HE Long, YU Qunli, ZHANG Yueyue, LI Ying, XU Jin, ZHANG Li, HAN Guangxing. Preparation and Structure-Activity Analysis of Antioxidant Peptides from Collagen by Ultra-high Pressure-Assisted Enzymatic Hydrolysis [J]. FOOD SCIENCE, 2026, 47(3): 109-119.
[11]	LIU Jiaxian, KAN Qixin, HU Wei, ZHOU Liang, GENG Pengfei, CAO Yong. Advances in Research on Egg Yolk Lecithin: Extraction, Identification, Bioactivities and Applications [J]. FOOD SCIENCE, 2026, 47(2): 386-397.
[12]	GUO Xingli, WANG Yuanhe, ZHANG Yanye, XU Pengcheng, ZHOU Ying, HU Jiangmiao, YANG Liu. Structural Characterization and in Vitro Hypoglycemic Activity of Polysaccharides from Dendrobium nobile Lindl. [J]. FOOD SCIENCE, 2026, 47(10): 85-96.
[13]	WANG Qi, WANG Jiayou, ZENG Jun, HUO Xiangdong, Razigul Kurban, SUN Jian, ZHAO Zhongkai, GAO Yan. Isolation and Characterization of Lactic Acid Bacteria from Traditional Xinjiang Fermented Foods and Untargeted Metabolomic Analysis of Xinjiang Cheese Fermented with Them [J]. FOOD SCIENCE, 2026, 47(10): 151-166.
[14]	HE Shanshan, LIU Liping, LIU Yanfang, FENG Jie, WANG Guangqiang, WANG Wenhan, ZHANG Jinsong. Bioactivity and Structural Characterization of Soluble Dietary Fiber from Lyophyllum decastes [J]. FOOD SCIENCE, 2025, 46(9): 39-47.
[15]	HAN Dong, ZHANG Jiahui, JIANG Zhengqiang, CHANG Chang. Structural Characterization and Anti-hyperuricemic Activity of Exopolysaccharides Secreted by Lactiplantibacillus plantarum-30 [J]. FOOD SCIENCE, 2025, 46(9): 148-155.

Purification, Identification and Characterization of Membrane-bound Alcohol Dehydrogenase from Gluconobacter oxydans DSM 2003

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments