Purification of Aucubin from the Crude Extract of Eucommia ulmoides Seeds by Silica Gel Chromatography

doi:10.7506/spkx1002-6630-201014013

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (14): 58-61.doi: 10.7506/spkx1002-6630-201014013

• Processing Technology • Previous Articles Next Articles

Purification of Aucubin from the Crude Extract of Eucommia ulmoides Seeds by Silica Gel Chromatography

LI Hui1,2，WANG Lan1，PENG Yu-dan1，ZHANG Yong-kang1,2

1. College of Biology and Environmental Science, Jishou University, Jishou 416000, China；2. Hunan Provincial Key Laboratory
of Forest Products and Chemical Industry Engineering, Jishou University, Zhangjiajie 427000, China

Received:2009-11-05 Revised:2010-04-01 Online:2010-07-15 Published:2010-12-29
Contact: LI Hui E-mail:lihuijsdx@163.com

Abstract

Abstract:

A silica gel chromatographic method was presented for the purification of aucubin (Au) from the crude extract of the residue of Eucommia ulmoides seed meal left after linolenic acid extraction. Subsequently, the extracted product was analyzed qualitatively and quantitatively. An optimal purification of Au was achieved by using a 2-fold mass of silica gel for Au adsorption followed by desorption with a 70% methanol/chloroform/petroleum ether/ethyl acetate mixture (7:1:0.5:1.5, V/V) at a flow rate of 1.0 mL/min. The eluate fractions were harvested and subjected to vacuum condensation, crystallization, filtration and recrystallization, and a final Au product with 96.56% purity was obtained.

Key words: aucubin, column chromatography, silica gel, purification

CLC Number:

LI Hui1,2，WANG Lan1，PENG Yu-dan1，ZHANG Yong-kang1,2. Purification of Aucubin from the Crude Extract of Eucommia ulmoides Seeds by Silica Gel Chromatography[J]. FOOD SCIENCE, 2010, 31(14): 58-61.

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Purification of Aucubin from the Crude Extract of Eucommia ulmoides Seeds by Silica Gel Chromatography

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