FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (14): 197-200.doi: 10.7506/spkx1002-6630-201014044

• Analysis & Detection • Previous Articles     Next Articles

Determination of Chlormequat Residues in Vegetables by High Performance Liquid Chromatography-Mass Spectrometry

ZHOU Yan-ming,HAN Yu,TIAN Hong-zhe,CHEN Bai   

  1. (Analysis and Testing Center, Shenyang Agricultural University, Shenyang 110161, China)
  • Received:2009-06-16 Revised:2009-12-02 Online:2010-07-15 Published:2010-12-29
  • Contact: ZHOU Yan-ming E-mail:zhouyanmingzym@163.com

Abstract:

A analytical method using high performance liquid chromatography (HPLC) combined with electrospray ionization mass spectrometry (ESI/MS) was developed for determining chlormequat residues in vegetables. Samples were extracted with methanol/water/acetic acid, and the extract was then centrifugated. The supernatant was harvested and cleaned up on a SPE-SCX column before HPLC-ESI/MS analysis. Retention times and characteristic ions were used for qualification and external standard method for quantification. A good linear of the calibration curve developed was exhibited over a concentration range of 0.03-10 μg/mL with a correlation coefficient of 0.9991. Average recoveries for chlormequat in 5 vegetables ranged from 74.92% to 103.28% with a relative standard deviation from 2.92% to 10.48%. The quantitative limit for chlormequat was 0.02 mg/kg.

Key words: vegetable, chlormequat, high performance liquid chromatography combined with electrospray ionization mass spectrometry

CLC Number: