Abstract:

A HPLC-MS/MS method based on QuEChERS extraction has been developed for determining cyclohexanediones herbicide residues in foodstuffs of animal origin. Cyclohexanediones herbicide residues in samples were extracted with acidic acetonitrile under the assistance of high-speed homogenization. The extract was cleaned up with N-propyl ethylenediamine (PSA), octadecylsilane (ODS) and graphitized carbon black prior to HPLC-MS/MS analysis. The calibration curves of 8 cyclohexanedione compounds developed all exhibited good linearity over a concentration range from 0.0025 to 0.1000 μg/mL, with a correlation coefficient ranging from 0.9973 to 0.9996. Average recoveries for the analytes in pork spiked at 0.005, 0.010 mg/kg and 0.050 mg/kg were between 75.68% and 106.80%, with a relative standard deviation between 6.65% and 11.04%. The detection limit was 0.005 mg/kg for all of the analytes.

Key words: cyclohexanediones herbicide, residual, LC-MS/MS, foodstuffs of animal origin