Abstract:

Objective: To develop an HPLC method for the simultaneous determination of several water-soluble vitamins in functional beverages. Methods: Luna C18(2) column (200 mm × 4.60 mm, 5μm) was used as stationary phase. The mobile phase was composed of 0.005 mol/mL hexanesulfonste sodium, methanol and glacial acetic acid (80: 20: 0.5) at pH 4.0. The flow rate was 1.0 mL/min. The wavelength of UV detection was set at 278 nm. Results: The standard curves developed exhibited a good linear over a range of 2.94－43.50μg/mL for VC, 6.00－90.00μg/mL for VPP, 1.70－25.50μg/mL for VB6, 1.60 －24.00μg/mL for VB2, and 2.30－43.50μg/mL for VB1. The detection limits for VC, VPP, VB6, VB2 and VB1 were 0.001, 0.362, 0.095, 0.328μg/mL and 0.619μg/mL, respectively. The average recoveries were 98.29% for VC, 98.38% for VPP and 98.51% for VB6. Conclusion: This method is simple, fast and accurate. It allows the simultaneous determination of water-soluble vitamins in functional beverage.

Key words: RP-HPLC, functional beverage, water-soluble vitamins, simultaneous determination