Enzymatic Synthesis of Caprylic Caprylate in an Organic Solvent

doi:10.7506/spkx1002-6630-201015010

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (15): 44-49.doi: 10.7506/spkx1002-6630-201015010

• Basic Research • Previous Articles Next Articles

Enzymatic Synthesis of Caprylic Caprylate in an Organic Solvent

ZHANG Feng-xiu1，ZHANG Guang-xian2,*

1. College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China；
2. College of Textile and Garment, Southwest University, Chongqing 400715, China

Received:2009-12-24 Revised:2010-05-15 Online:2010-08-15 Published:2010-12-29
Contact: ZHANG Guang-xian2 E-mail:zgx656472@sina.com

Abstract

Abstract:

The synthesis of caprylic caprylate catalyzed by lipase from porcine pancreas in an organic solvent was carried out to explore the effects of organic solvent type, reaction temperature, amount of added water, reaction time, lipase amount, substrate concentration and caprylic acid-to-caprylic alcohol ratio on caprylic acid conversion. The optimum organic solvent for caprylic caprylate synthesis was n-hexane. Reaction temperature of 45 ℃, amount of added water of 29μL, caprylic acid-tocaprylic alcohol ratio of 1:1.2－1:1.5, caprylic acid concentration of 0.23－0.33 mol/L, lipase concentration of 2.314 g/L were found optimum. Under these optimized conditions, the conversion ratio of caprylic acid was 98.12%. In addition, the structure of synthesized product was characterized by IR, 1H NMR and 13C NMR. This synthesis method can yield a high conversion ratio of caprylic acid under mild reaction conditions.

Key words: lipase, synthesis, caprylic caprylate, organic solvent, esterification

CLC Number:

TS225 3

ZHANG Feng-xiu1，ZHANG Guang-xian2,*. Enzymatic Synthesis of Caprylic Caprylate in an Organic Solvent[J]. FOOD SCIENCE, 2010, 31(15): 44-49.

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Enzymatic Synthesis of Caprylic Caprylate in an Organic Solvent

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