FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (17): 323-326.doi: 10.7506/spkx1002-6630-201017072

• Bioengineering • Previous Articles     Next Articles

Screening of Optimal Liquid Medium for Hypsizigus marmoreus and Optimization of Its Shake Flask Incubation Conditions

WANG Li-juan，LIU Lin-de，BU Qing-mei，WANG Ai-yun，WANG Shu-fang   

  1. School of Life Sciences, Ludong University, Yantai 264025, China
  • Received:2010-06-27 Online:2010-09-15 Published:2010-12-29
  • Contact: WANG Li-juan E-mail:wanglijuan53@126.com

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Abstract:

In this study, medium composition and process conditions for the liquid-state incubation of Hypsizigus marmoreus in a shake flask were explored. The optimal medium composition was found to consist of 3 g/100 mL glucose, 1 g/100 mL corn starch, 1.5 g/100 mL soybean cake, 0.3 g/100 mL (NH4)2SO4, 0.2 g/100 mL KH2PO4, 0.1 g/100 mL MgSO4·7H2O and 10 mg/L Vitamin B1. Initial pH of 6.0, medium volume contained in a 500 mL flask of 140－150 mL, shaking speed of 180 r/min, incubation temperature of 26 ℃, incubation time of 72 h and inoculum size of 10%－12% were found optimal.

Key words: Hypsizigus marmoreus, medium selection, incubation conditions

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