Abstract:

Acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC) were extracted from the scales of grass carp at low temperature, and optimal extraction parameters were explored. The optimal parameters for removing impurity proteins from fish scales with 0.1 mol/L Na2CO3 were material/liquid ratio of 1:40 for mixing 3 times for 6 h each time at room temperature. The optimal parameters for removing mineral impurities with 0.3 mol/L EDTA were material/liquid ratio of 1:60 for mixing 3 times 12 h for each time at room temperature. The optimal parameters for ASC extraction were material/liquid ratio of 1:60 for extraction with 0.8 mol/L acetic acid 3 times for 24 h each time at 25 ℃. The optimal parameters for PSC extraction were amount of pepsin on the basis of the weight of fish scales, 3.0%; acetic acid concentration, 0.8 mol/L; and material/liquid ratio 1:30 for twice extraction at 4 ℃ for 24 h each time.

Key words: the scales of grass carp, collagen, extraction, optimal parameters