FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (18): 244-247.doi: 10.7506/spkx1002-6630-201018056

• Analysis & Detection • Previous Articles     Next Articles

Determination of Six Acaricide Residues in Beeswax by Gas Chromatography Coupled with a Micro-electron Capture Detector

CHEN Fang1，LI Dan2，ZHANG Jin-zhen1，HUANG Jing-ping1，ZHAO Jing1，WU Li-ming1,*   

  1. 1. Apiculture Sub-Centers, National Agricultural Products Processing Center, Bee Research Institute, Chinese Academy of Agricultural
    Sciences, Beijing 100093, China；2. Biological Medicine Department, Beijing City University, Beijing 100083, China
  • Received:2010-02-23 Online:2010-09-25 Published:2010-12-29
  • Contact: WU Li-ming1,* E-mail:apiswu@126.com

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Abstract:

An accurate, fast and convenient method for simultaneous determination of 6 acaricide residues in beeswax by gas chromatography coupled with a micro-electron capture detector (GC-MECD) was established. The sample was saturated with hexane and acetonitrile in water bath with constant temperature. After frozen and centrifugation at 10000 r/min, the extract was analyzed by GC-MECD. Effects of extraction solvent, solvent volume and extraction times on extraction efficiency were explored. The best extraction solvent was 30.0 mL of acetonitrile-hexane (V:V, 2:1). Twice freezing and high speed centrifugation could remove the interference of lipid matrix. This method exhibited an excellent linear relationship over the range of 0.01 to 4.0 mg/L with a correlation coefficient of larger than 0.9878. The limits of detection (LOD) for the 6 acaricides varied from 0.96 to 4.69 μg/kg. The average recovery rates of these analytes spiked at three different levels ranged from 83.8% to110.4%, and the relative standard deviations (RSD) from 1.70% to 6.60%.

Key words: acaricide, beeswax, gas chromatography coupled with a micro-electron capture detector (GC-MECD)

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