Abstract:

The contamination of Helicobacter pylori is considered as a serious problem to impair public health in both developed and developing countries. In order to establish a fast, specific and sensitive method to detect the contamination of Helicobacter pylori in food, PCR method was used to detect the DNA of Helicobacter pylori based on urease gene. Results indicated that this method was highly specific to Helicobacter pylori and sensitive and presented a limit detection of 8 CFU/mL for Helicobacter pylori. Therefore, this method is applicable to clinical practice, environment control and inspection of exported food.

Key words: Helicobacter pylori, urease gene, PCR, detection