Abstract:

Polyclonal antibodies against fumonisin B1 were produced in rabbits with FB1/keyhole limpet hemocyanin conjugate. A sensitive indirect competitive enzyme-linked immunosorbent assay (ELISA) for determining FB1 was developed. The concentration of FB1 causing 50% inhibition of binding enzyme marker (IC50) was 11.7μg/L and the detection limit was 1.1μg/L. The average variation coefficients in intragroup and intergroup were 5.8% and 10.2%, respectively. PBS was selected as the best extract solvent through extensive investigations. The recovery rates from corn, wheat, rice and sorghum samples spiked at a level varying from 50 to 500μg/kg ranged from 70.5% to105.6%. The contents of FB1 in 40 cereal samples were screened by the developed ELISA method and the results exhibited a positive correlation with the results from HPLC (R2 = 0.9547). This method is sensitive, fast and accurate and therefore is suitable for screening the presence of FB1 in cereals.

Key words: fumonisin B1, ELISA, cereal