FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (19): 212-215.doi: 10.7506/spkx1002-6630-201019046

• Bioengineering • Previous Articles     Next Articles

Screening of an Optimal Strain for Producing Rapeseed-originated Angiotensin I-converting Enzyme Inhibitory Peptides by Liquid-state Fermentation

JU Xing-rong1,JIN Jing1,YUAN Jian1,WANG Li-feng1,2,HE Rong1,2   

  1. 1. College of Food Science and Engineering, Nanjing University of Finance and Economics, Key Laboratory of Grain and Oils Quality
    Control and Deep-Utilizing Technology of Jiangsu Province, Nanjing 210003, China;2. School of Food Science and Technology,
    Jiangnan University, Wuxi 214122, China
  • Received:2010-01-11 Revised:2010-07-19 Online:2010-10-15 Published:2010-12-29
  • Contact: JU Xing-rong1 E-mail:xingrongju@163.com

Abstract:

In order to search for an optimal stain for the production of angiotensin I-converting enzyme (ACE) inhibitory peptides from rapeseed meal by liquid-state fermentation, seven common microorganisms including Bacillus subtilis, Bacillus licheniformis, Aspergillus niger, Geotrichum candidum, Candida utilis, Actinomucor elegans and Aspergillus oryzae as a starter for rapeseed meal fermentation were compared for their differences in five fermentation parameters, namely peptide yield, nitrogen solubility index (NSI), amino nitrogen yield, soluble nitrogen in aqueous trichloroacetic acid (SN-TCA) content and ACE inhibition activity. Among the above microorganisms, Bacillus subtilis was found to give both the highest peptide yield and ACE inhibition activity and grow dominantly, thereby being a superior strain for the production of ACE inhibitory peptides from rapeseed meal by liquid-state fermentation.

Key words: rapeseed-derived peptide, liquid-state fermentation, strain screening, ACE inhibitory peptide

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