Abstract:

The conditions for extracting intracellular polysaccharides from the fermented mycelia of Fomes officinalis Ames that is a traditional Uyghur medicine in Xinjiang autonomous region and extracting extracellular polysaccharides from the fermentation supernatant of the strain were optimized using single factor and orthogonal array design methods. The optimal conditions for the extraction of intracellular polysaccharides under the assistance of ultrasonic were determined to be: ultrasonic power 90 W; ultrasonic treatment time, 15 min; hot water temperature, 90 ℃; dried mycelia/distilled water ratio, 1:20; and length of extraction time, 3 hours, and an intracellular polysaccharide yield of 66.4 mg/g dried mycelia was obtained under such conditions. The optimal extraction process of extracellular polysaccharides consisted of adjusting the pH of the fermentation supernatant to 7 and adding 60% ethanol for polysaccharide precipitation for 24 hour at 4 ℃, and the resultant polysaccharide yield was 92.1%.

Key words: Fomes officinalis Ames, fermentation, polysaccharide, extraction