Separation and Purification of Monascus Pigments by High-speed Counter-current Chromatography

doi:10.7506/spkx1002-6630-201020039

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (20): 192-195.doi: 10.7506/spkx1002-6630-201020039

• Processing Technology • Previous Articles Next Articles

Separation and Purification of Monascus Pigments by High-speed Counter-current Chromatography

ZHENG Yun-quan1，LI Yong-ning1，WANG A-wan2，CHEN Fen-ling2，SHI Xian-ai2，GUO Yang-hao1,2,*

1. Institute of Pharmaceutical Biotechnology and Engineering, Fuzhou University, Fuzhou 350002, China；
2. Fujian Key Laboratory of Medical Instrumentation and Pharmaceutical Technology, Fuzhou 350002, China

Received:2010-05-21 Revised:2010-09-19 Online:2010-10-25 Published:2010-12-29
Contact: GUO Yang-hao E-mail:yanghaoguo@yahoo.com.cn

Abstract

Abstract:

Six azaphilone-type pigments were separated from Monascus-fermented solid medium and purified/fractionated by by high-speed counter-current chromatography (HSCCC). A solvent system with weak polarity involving four components nhexane, ethyl acetate, methanol and water was selected and used for HSCCC. Based on a comparative analysis of partition coefficients of target compounds in different solvent systems, a two-step HSCCC routine was developed. After separation by HSCCC and crystallization with acetone, six azaphilone-type pigments with high purity were obtained. The purity of each pigment was above 98.5% and their yields were between 81.40% and 84.78%. Their molar absorption coefficients were 13313, 13877, 9380, 9360, 25621 L/(mol·cm) and 25849 L/(mol·cm), separately.

Key words: Monascus pigments, high-speed counter-current chromatography, separation and purification

CLC Number:

TS202
Q819

ZHENG Yun-quan1，LI Yong-ning1，WANG A-wan2，CHEN Fen-ling2，SHI Xian-ai2，GUO Yang-hao1,2,*. Separation and Purification of Monascus Pigments by High-speed Counter-current Chromatography[J]. FOOD SCIENCE, 2010, 31(20): 192-195.

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Separation and Purification of Monascus Pigments by High-speed Counter-current Chromatography

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