FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (20): 275-277.doi: 10.7506/spkx1002-6630-201020055

• Analysis & Detection • Previous Articles     Next Articles

Application of High Performance Capillary Electrophoresis to the Determination of Flavonoid Compounds

ZHOU Yi-ming1，ZHOU Xiao-li1,*，CUI Lin-lin2   

  1. 1. School of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai 200235, China；
    2. College of Food Science, Shanghai Business School, Shanghai 200235, China
  • Received:2009-12-03 Revised:2010-06-21 Online:2010-10-25 Published:2010-12-29
  • Contact: ZHOU Xiao-li E-mail:zhouxl@sit.edu.cn

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Abstract:

A method was developed for the determination of flavonoid compounds using high performance capillary electrophoresis (HPCE). The electrolyte buffer used was 20 mmol/L borax/boracic acid solution (pH 8.4). Electrophoresis was performed at 25 ℃ and 20 kV voltage. The detection wavelength was set as 245 nm. The developed method displayed good linearity. A thorough chromatographic separation of flavonoid compounds was achieved within 10 min, which meets the requirements for qualification and quantification. This method giving good separation and having high precision is applicable for the determination of flavonoid compounds in practice.

Key words: flavonoids, high performance capillary electrophoresis (HPCE)

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