Abstract:

A simultaneous determination method for nine kinds of mycotoxins in maize, peanut and wheat was developed using ultra-high performance liquid chromatography-tendem mass spectrometry (UPLC-MS/MS). Samples were sequentially extracted with PBS solution followed by 70% methanol, and then the extracts were pooled and cleaned up with immunoaffinity column, followed by blowing to dryness under nitrogen gas stream, reconstitution with acetonitrile/water mixture before the analysis on UPLC-MS/MS. The MS conditions used were negative (ESI+) and positive (ESI－) electrospray ionization and MRM models. The results indicated as follows: 1) all the nine kinds of mycotoxins had good linear relationship with peak area over the range from 0.1 to 400μg/kg, and the detection limits for them ranged from 0.1 to 20μg/kg; and 2) The average recovery rates in the above three matrices spike at three levels were between 59.5% and 118.8%, with a relative standard deviation (RSD) varying from 2.4% to 12.8%. This analytical method meets the requirements for trace assays. In addition, it is simple, rapid, accurate and suitable for the simultaneous determination of nine kinds of mycotoxins in maize, peanut and wheat.

Key words: UPLC-MS/MS, mycotoxin, maize, peanut, wheat