Establishment of Loop-mediated Isothermal Amplification for the Detection of Streptococcus hemolyticus

doi:10.7506/spkx1002-6630-201022067

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (22): 311-314.doi: 10.7506/spkx1002-6630-201022067

• Analysis & Detection • Previous Articles Next Articles

Establishment of Loop-mediated Isothermal Amplification for the Detection of Streptococcus hemolyticus

YIN Huan，LI Qi，CHEN Jiang-yuan，MA Yin-zhong，LI Rui，LIU Zhi-guo*

College of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, China

Received:2010-06-27 Revised:2010-10-11 Online:2010-11-25 Published:2010-12-29
Contact: LIU Zhi-guo E-mail:zhiguo_1@126.com

Abstract

Abstract:

Objective: To develop a loop-mediated isothermal amplification (LAMP) method for the rapid detection of Streptococcus hemolyticus. Methods: According to the scpA gene of Streptococcus hemolyticus, 4 specific primers were designed for LAMP amplification. Six different Streptococcus hemolyticus strains were validated by LAMP and identified by enzymatic digestion. The sensitivity of LAMP method was evaluated by serial gradient dilution of Streptococcus hemolyticus solution. Results: LAMP could be used for the rapid and sensitive detection of Streptococcus hemolyticus with a high sensitivity and a detection limit of 16.7 CFU/mL. Moreover, the detection limit was 10 CFU/mL in milk samples. Conclusion: LAMP method is a simple, rapid and feasible method for the detection of Streptococcus hemolyticus.

Key words: Streptococcus hemolyticus, loop-mediated isothermal amplification, detection, detection limit

CLC Number:

Q93.3

YIN Huan，LI Qi，CHEN Jiang-yuan，MA Yin-zhong，LI Rui，LIU Zhi-guo*. Establishment of Loop-mediated Isothermal Amplification for the Detection of Streptococcus hemolyticus[J]. FOOD SCIENCE, 2010, 31(22): 311-314.

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Establishment of Loop-mediated Isothermal Amplification for the Detection of Streptococcus hemolyticus

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