Development of a Duplex PCR for Detection of Salmonella spp and Campylobacter jejuni in Food

doi:10.7506/spkx1002-6630-201022089

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (22): 403-406.doi: 10.7506/spkx1002-6630-201022089

• Analysis & Detection • Previous Articles Next Articles

Development of a Duplex PCR for Detection of Salmonella spp and Campylobacter jejuni in Food

CHEN Nuo1，TANG Shan-hu1,*，CHEN Jin-hui2，CEN Lu-jia1，LI Xue1，LONG Hu1

1. College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China；
2. Dongguan Inspection and Quarantine Bureau, Dongguan 523072, China

Received:2010-07-07 Revised:2010-10-21 Online:2010-11-25 Published:2010-12-29
Contact: TANG Shan-hu E-mail:stang01@126.com

Abstract

Abstract:

A rapid assay for simultaneous detection of Salmonella spp and Campylobacter jejuni in food was developed. The primers were designed based on the fimY gene of Salmonella spp and hipO gene of Campylobacter jejuni. Annealing temperatures, Mg2+ concentrations, and primer concentrations were optimized. The detection limit was 3.98 pg for Salmonella spp and 4.05 pg for Campylobacter jejuni using the single PCR method. However, the detection limit of the duplex PCR method was significantly higher, which was 398 pg, 40.5 pg for Salmonella spp and Campylobacter jejuni, respectively. In this study, a duplex PCR with high specificity and sensitivity was developed and it would provide useful information for the simultaneous detection of Salmonella spp and Campylobacter jejuni in food.

Key words: Salmonella spp, Campylobacter jejuni, Duplex PCR, Detection

CLC Number:

S855.11

CHEN Nuo1，TANG Shan-hu1,*，CHEN Jin-hui2，CEN Lu-jia1，LI Xue1，LONG Hu1. Development of a Duplex PCR for Detection of Salmonella spp and Campylobacter jejuni in Food[J]. FOOD SCIENCE, 2010, 31(22): 403-406.

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Development of a Duplex PCR for Detection of Salmonella spp and Campylobacter jejuni in Food

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