Biological Characteristics of Lytic SF-A2 Phage and Its Sterilization Effect in Milk

doi:10.7506/spkx1002-6630-201023048

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (23): 214-218.doi: 10.7506/spkx1002-6630-201023048

• Bioengineering • Previous Articles Next Articles

Biological Characteristics of Lytic SF-A2 Phage and Its Sterilization Effect in Milk

ZHANG Hui，WANG Ran*，BAO Hong-duo

Key Laboratory of Agro-Food Safety and Quality, Ministry of Agriculture, Key Laboratory of Food Quality and Safety of Jiangsu
Province-State Key Laboratory Breeding Base, Key Laboratory of Animal-derived Food Safety of Jiangsu Province, Jiangsu Academy of
Agricultural Sciences, Nanjing 210014, China

Received:2010-09-19 Online:2010-12-15 Published:2010-12-29
Contact: WANG Ran E-mail:wangran2001@126.com

Abstract

Abstract:

In order to analyze biological characteristics of Shigella flexneri (S. flexneri) phage and its sterilization effect in milk, the lytic S. flexneri phage SF-A2 was isolated from sewage. Electron microscope observation showed that purified phage SF-A2 was a member of family Myoviridae. Based on its biological characteristic assay, phage SF-A2 exhibited the tolerance to temperature and pH. In addition, phage SF-A2 could effectively inactivate S. flexneri. After adding phage SF-A2 for 48 h, the concentration of S. flexneri was reduced by 103 folds compared with the host control. After 72 h incubation, the concentration of S. flexneri was declined to the limit of detection. Therefore, SF-A2 is a virulent bacteriophage and can effectively inactivate S. flexneri in ready-to-eat milk. It will be a good biological control agent for food safety and also an efficient antimicrobial source for diarrhea.

Key words: Shigella flexneri, bacteriophage, biological control

CLC Number:

ZHANG Hui，WANG Ran*，BAO Hong-duo. Biological Characteristics of Lytic SF-A2 Phage and Its Sterilization Effect in Milk[J]. FOOD SCIENCE, 2010, 31(23): 214-218.

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Biological Characteristics of Lytic SF-A2 Phage and Its Sterilization Effect in Milk

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