Abstract:

In the present study, the recombinant endolysin from Salmonella-infecting bacteriophase STP4-a was
characterized for its biochemical properties such as pH, temperature and iron concentration by the plate diffusion and
turbidity methods. The results showed that the endolysin was stable over broad pH and temperature ranges and was active
from pH 5.0 to 10 and from 30 to 50 ℃. After 6 months of storage at − 80 ℃, the activity of endolysin retained 85% of the
original level. Ethylenediaminetetraacetic acid (EDTA) was used to increase outer membrane permeability, and it greatly
enhanced the lytic activity of STP4-a endolysin.

Key words: Salmonella, lytic bacteriophage, endolysin