Interdelta PCR Fingerprinting for the Identification of Saccharomyce cerevisiae from Shacheng Area

doi:10.7506/spkx1002-6630-201023062

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (23): 281-284.doi: 10.7506/spkx1002-6630-201023062

• Bioengineering • Previous Articles Next Articles

Interdelta PCR Fingerprinting for the Identification of Saccharomyce cerevisiae from Shacheng Area

ZHAO Jing-jing1，LI Yan1,2,*，ZHANG Li-zhong3，ZHUANG Yu-ting1

1. College of Bioscience and Bioengineering, Hebei University of Science and Technology, Shijiazhuang 050018, China；
2. R&D Center for Fermentation Engineering of Hebei Province, Shijiazhuang 050018, China；
3. China Great Wall Wine Co. Ltd., Shacheng 075400, China

Received:2010-07-06 Revised:2010-11-22 Online:2010-12-15 Published:2010-12-29
Contact: LI Yan E-mail:lymdh5885@163.com

Abstract

Abstract:

Interdelta PCR fingerprinting technology was used to differentiate 54 Saccharomyce cerevisiae strains isolated from vineyard soil, brewing equipments and spontaneous fermentation process of Longan grape in Shacheng area. Results indicated that 7 genetic patterns were distinguished by Interdelta PCR fingerprinting. The genetic patterns I through VII accounted for 31.5%, 11.1%, 7.4%, 42.6%, 3.7%, 1.85% and 1.85%, respectively. The numbers of genotypes of yeasts isolated the late stage of spontaneous fermentation, the surface of brewing equipments and vineyard soil were 4, 3 and 2, respectively. Based on the cluster analysis tree, the genetic pattern I and IV had the closest genetic relationship, and the genetic pattern VII had the most far genetic relationship with others. The distribution and variation discipline of different Saccharomyce cerevisiae strains will provide a theoretical foundation for choosing excellent strains with their own favor characteristics.

Key words: Saccharomyce cerevisiae, Interdelta PCR, differentiation and identification, cluster analysis, Shacheng area, Longan grape

CLC Number:

Q93.331

ZHAO Jing-jing1，LI Yan1,2,*，ZHANG Li-zhong3，ZHUANG Yu-ting1. Interdelta PCR Fingerprinting for the Identification of Saccharomyce cerevisiae from Shacheng Area[J]. FOOD SCIENCE, 2010, 31(23): 281-284.

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Interdelta PCR Fingerprinting for the Identification of Saccharomyce cerevisiae from Shacheng Area

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