Abstract:

Chlorogenic acid was extracted from the fruit of Chaenomeles sinensis Koehne using methanol as solvent. Effects of four extraction variables (methanol concentration, extraction temperature, solid/liquid ratio and extraction time) on yield of chlorogenic acid were investigated by conducting single factor test. Subsequently, a L9(34) orthogonal test was employed to optimize the extraction conditions. In the following, the anti-Shigella dysenteriae and Staphylococcus aureus activities were evaluated by using inhibition zone test. Results showed the optimum extractions conditions were as follows: aqueous methanol concentration 85%, the solid/liquid ratio 1:30 (C. sinensis Koehne powder(g)/aqueous methanol (mL)), extraction at 40 ℃ for 14 hours. Under the optimum conditions, the yield of chlorogenic acid were 0.142%. The antibacterial activity of chlorogenic acid samples against S. dysenteriae and S. aureus was significant. It indicated that chlorogenic acid may be a main pharmacological compound of a folk medicine of white spirit soaked with C. sinensis Koehne.

Key words: Chaenomeles sinensis Koehne, chlorogenic acid, extraction and optimization, antibacterial activity, Shigella dysenteriae, Staphylococcus aureus.