Preparation Processing of Ultrafine Papain Particle by Supercritical Carbon Dioxide Anti-solvent Method

doi:10.7506/spkx1002-6630-201024014

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (24): 70-73.doi: 10.7506/spkx1002-6630-201024014

• Processing Technology • Previous Articles Next Articles

Preparation Processing of Ultrafine Papain Particle by Supercritical Carbon Dioxide Anti-solvent Method

ZHAO Liang-zhong1 ZENG Xiang-yan1 CHEN Jian1 CHEN Ling-juan2 XU Xiao-dong3

1. Department of Chemical Biotechnology ang Engineering, Shaoyang University, Shaoyang 422000, China
2. College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China
3. Haian Huada Oil Instrument Co. Ltd., Nantong 226600, China

Received:2010-09-09 Revised:2010-11-16 Online:2010-12-25 Published:2010-12-29
Contact: ZHAO Liang-zhong E-mail:sys169@163.com

Abstract

Abstract:

Supercritical carbon dioxide anti-solvent crystallization technology was used to prepare ultrafine papain particles to decide the optimal solvent type and solvent volume. Results indicated that the yield of ultrafine papain particles was positively correlated with solvent volume and enzyme activity was negatively correlated with solvent volume at the conditions of DMSO as the solvent. When dimethylformamide was used as the solvent, the yield of papain particles was low, but the high activity of papain particles was observed. The highest activity of papain particles was achieved to be 3484.691 U/mg at the condition of 70% solvent, which is much higher than the original activity of papain (400 U/mg). The ultrafine papain particles could not form when glycerol was used as the solvent.

Key words: supercritical, anti-solvent crystallization, ultrafine particle papain, enzyme activity

CLC Number:

Q556.3

ZHAO Liang-zhong1 ZENG Xiang-yan1 CHEN Jian1 CHEN Ling-juan2 XU Xiao-dong3. Preparation Processing of Ultrafine Papain Particle by Supercritical Carbon Dioxide Anti-solvent Method[J]. FOOD SCIENCE, 2010, 31(24): 70-73.

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Preparation Processing of Ultrafine Papain Particle by Supercritical Carbon Dioxide Anti-solvent Method

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