Iodometric Determination of 2-Keto-D-gluconic Acid in Fermentation Broth

doi:10.7506/spkx1002-6630-201024068

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (24): 314-317.doi: 10.7506/spkx1002-6630-201024068

• Analysis & Detection • Previous Articles Next Articles

Iodometric Determination of 2-Keto-D-gluconic Acid in Fermentation Broth

FENG Xiao-yan1，ZHOU Yan-zheng2，SUN Wen-jing1,2，WANG Da-ming1，YU Si-lian3，LIU Jing-ze1,*

1. College of Life Science, Hebei Normal University, Shijiazhuang 050016, China；2. School of Food and Biological Engineering,
Jiangsu University, Zhenjiang 212013, China；3. Parchn Sodium Isovitamin C Co. Ltd., Dexing 334221, Chi

Received:2010-08-30 Revised:2010-11-02 Online:2010-12-25 Published:2010-12-29
Contact: LIU Jing-ze1 E-mail:liujingze@mail.hebtu.edu.cn

Abstract

Abstract:

Iodometric method was improved to prevent the interference of glucose in determination of 2-keto-D-gluconic acid (2KGA) in fermentation broth. The results revealed a good linear relationship among the volumes of I2 standard solution consumed and concentrations of 2-keto-D-gluconic acid and glucose (R2＞0.9999). The average recovery was 95.68% with the relative standard deviation (RSD) of 1.35% (n=5). The method developed here had good accuracy, stability and reproducibility, and was easily operated, hereby could be used for the determination of concentrations of 2-keto-D-gluconic acid in fermentation broth.

Key words: 2-keto-D-gluconic acid, glucose, fermentation broth, iodometry

CLC Number:

TQ921.2

FENG Xiao-yan1，ZHOU Yan-zheng2，SUN Wen-jing1,2，WANG Da-ming1，YU Si-lian3，LIU Jing-ze1,*. Iodometric Determination of 2-Keto-D-gluconic Acid in Fermentation Broth[J]. FOOD SCIENCE, 2010, 31(24): 314-317.

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Iodometric Determination of 2-Keto-D-gluconic Acid in Fermentation Broth

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