Abstract:

Effect of the extraction method, solvent, solvent concentration, pH and time on the yield of vitamin B2 in the millet was studied through single factor test and orthogonal design test. Vitamin B2 content was determined by fluorophotometric method with excitation and emission wavelength at 471.0 nm and 528.0 nm, respectively. In the optimum extraction conditions, millet was extracted with 0.1 mol/L of aqueous H3PO4 at pH 5.0 for 45 min in a water bath backflow. Under such conditions, the recovery of VB2 was from 89.20% to 100.32%, with the coefficient of variation 1.21%.

Key words: millet, vitamin B2(VB2), orthogonal experiment, fluorophotometry