Synthesis and Identification of the Artificial Antigens for Sunset Yellow and Tartrazine

doi:10.7506/spkx1002-6630-201101030

FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (1 ): 124-126.doi: 10.7506/spkx1002-6630-201101030

• Bioengineering • Previous Articles Next Articles

Synthesis and Identification of the Artificial Antigens for Sunset Yellow and Tartrazine

LIN Fei，LIU Li-qiang，KUANG Hua，PENG Chi-fang，SONG Shan-shan，HU Jin-wei，XU Chuan-lai*

School of Food Science and Technology, Jiangnan University, Wuxi 214122, China

Received:2010-07-16 Online:2011-01-15 Published:2010-12-28
Contact: XU Chuan-lai E-mail:xcl@jiangnan.edu.cn

Abstract

Abstract:

This study was aimed to develop suitable complete antigens based on either sunset yellow or tartrazine, two pigment compounds. Based on the chemical structural characteristics of the target molecules, we used various activation methods including succinic anhydride and mixed anhydride to acquire complete antigens. Bovine serum albumin (BSA) was served as the carrier protein to couple with the haptens. The results from ultra-violet spectral and high perform liquid chromatography-mass spectrometry analyses showed that the immunogen based on tartrazine was synthesized successfully. The coupling ratio of tartrazine to the carrier protein was 12, while that was only 6 for sunset yellow-BSA conjugate. The tartrazine-BSA conjugate prepared in this study can be used to induce the generation of the specific antibodies against tartrazine in animals.

Key words: sunset yellow, tartrazine, artificial antigens, synthesis

CLC Number:

TS202.3

LIN Fei，LIU Li-qiang，KUANG Hua，PENG Chi-fang，SONG Shan-shan，HU Jin-wei，XU Chuan-lai*. Synthesis and Identification of the Artificial Antigens for Sunset Yellow and Tartrazine[J]. FOOD SCIENCE, 2011, 32(1 ): 124-126.

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Synthesis and Identification of the Artificial Antigens for Sunset Yellow and Tartrazine

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