Abstract:

The key conditions for extracting total flavones from the branches and leaves of Sabina pingii var. wilsonii with aqueous ethanol solution under the assistance of ultrasound, including ultrasound treatment time, ethanol concentration, liquid/solid ratio, and extraction times, were optimized by combined single factor and orthogonal array design methods. The optimal conditions for improved extraction of total flavones were determined as follows: 70 min ultrasound treatment repeated twice for extracting the raw material with 10-fold volume (mL/g) of 75% aqueous ethanol solution. The maximum yield of total flavones was 5.29% under the optimized conditions.

Key words: Sabina pingii var. wilsonii, total flavones, ultrasound-assisted extraction, yield