Abstract:

Two types of macroporous resins, D101 and AB-8, were comparatively studied for their static adsorption and desorption performances towards sea asparagus flavonoids, and AB-8 was the selected resin for the separation and purification of the crude ethanol extract (rich in flavonoids) from sea asparagus prepared with microwave assistance. The optimum process for the adsorption of sea asparagus flavonoids onto AB-8 was that the crude extract was adjusted to a concentration of 0.5 mg/mL at pH 6 before loading at a flow rate of 1 mL/min. The flowing of 75% ethanol aqueous solution through AB-8 column at a rate of 2 mL/min was found optimum for the desorption of sea asparagus flavonoids, resulting in a desorption rate of 85.25%. The adsorption/desorption process is ease to operate and displays a good separation effect, thereby being suitable for the industrial separation and purification of sea asparagus flavonoids.

Key words: sea asparagus, flavonoids, macroporous resin