Determination of Molecular Weight Distribution of Garlic Oligosaccharide and Polysaccharide by Matrix-assisted Laser Desorption/Ionization Mass Spectrometry

doi:10.7506/spkx1002-6630-201102034

FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (2 ): 146-149.doi: 10.7506/spkx1002-6630-201102034

• Analysis & Detection • Previous Articles Next Articles

Determination of Molecular Weight Distribution of Garlic Oligosaccharide and Polysaccharide by Matrix-assisted Laser Desorption/Ionization Mass Spectrometry

HUANG Xue-song，YAN Feng-chao，WU Jian-zhong

Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China

Received:2010-02-23 Online:2011-01-25 Published:2011-01-10
Contact: HUANG Xue-song E-mail:thxs@jnu.edu.cn

Abstract

Abstract: A series of garlic oligosaccharide and polysaccharide samples were prepared by precipitation with varying volumes of acetone or ethanol, and their molecular weight distributions were determined by matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Three-fold volume of acetone could precipitate more oligosaccharide and polysaccharide than 4-fold volume of ethanol. The molecular weight distribution of oligosaccharide and polysaccharide was from 362 to 2811 u, and can be adjusted by applying different proportions of precipitators.

Key words: garlic (Allium sativum L.), oligosaccharide, polysaccharide, MALDI-TOF-MS, molecular weight distribution

CLC Number:

O629.12

HUANG Xue-song，YAN Feng-chao，WU Jian-zhong. Determination of Molecular Weight Distribution of Garlic Oligosaccharide and Polysaccharide by Matrix-assisted Laser Desorption/Ionization Mass Spectrometry[J]. FOOD SCIENCE, 2011, 32(2 ): 146-149.

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Determination of Molecular Weight Distribution of Garlic Oligosaccharide and Polysaccharide by Matrix-assisted Laser Desorption/Ionization Mass Spectrometry

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