Purification of Triterpenoids from Cyclocarya paliurus Leaves by Macro-porous Resin Adsorption

doi:10.7506/spkx1002-6630-201106014

FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (6 ): 61-65.doi: 10.7506/spkx1002-6630-201106014

• Processing Technology • Previous Articles Next Articles

Purification of Triterpenoids from Cyclocarya paliurus Leaves by Macro-porous Resin Adsorption

YIN Zhong-ping1,2，SHANGGUAN Xin-chen1,2,*，ZHANG Yue-hong2，LI Dong-ming2，WU Shao-fu2，CHEN Ji-guang2

1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China；
2. Laboratory of Plant Resources Exploitation and Utilization, Jiangxi Agricultural University, Nanchang 330045, China

Received:2010-05-19 Revised:2011-01-14 Online:2011-03-25 Published:2011-03-03
Contact: YIN Zhong-ping1 E-mail:yinzp2008@yahoo.com.cn

Abstract

Abstract: In order to obtain triterpenoids with high purity from Cyclocarya paliurus leaves, macro-porous resin was used for purification. Results indicated that macro-porous resin revealed a static absorption mode. AB-8 resin had good adsorption capability on triterpenoids from Cyclocarya paliurus leaves, and its adsorption capacity, desorption rate and total recovery rate were 77.49 mg/g, 85.44% and 72.01%, respectively. Dynamic adsorption and desorption experiments of AB-8 resin suggested that the increasing flow rate of sample solution could result in the decrease of adsorption rate, and the increasing sample amount in resin could result in a pattern of initial increase and final decline for adsorption rate. A better elution efficiency was observed at the concentration of 95% ethanol, and 10 BV was reasonable dosage. Both peak concentration and desorption rate were declined with the increasing flow rate of eluent. The best flow rate was 1.5 mL/min. The purity of triterpenoids purified by AB-8 macro-porous resin was 44.30%.

Key words: Cyclocarya paliurus, total triterpenoids, macro-porous resin, purification

CLC Number:

TQ028.15

YIN Zhong-ping1,2，SHANGGUAN Xin-chen1,2,*，ZHANG Yue-hong2，LI Dong-ming2，WU Shao-fu2，CHEN Ji-guang2. Purification of Triterpenoids from Cyclocarya paliurus Leaves by Macro-porous Resin Adsorption[J]. FOOD SCIENCE, 2011, 32(6 ): 61-65.

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Purification of Triterpenoids from Cyclocarya paliurus Leaves by Macro-porous Resin Adsorption

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