Determination of GSH in Lycium barbarum L Fruits by Derivatizing Agent

doi:10.7506/spkx1002-6630-201106058

FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (6 ): 250-255.doi: 10.7506/spkx1002-6630-201106058

• Analysis & Detection • Previous Articles Next Articles

Determination of GSH in Lycium barbarum L Fruits by Derivatizing Agent

ZHU Ya-ling，ZHANG Xiao-yong，CUI Sheng-yun*

Key Laboratory of Natural Resources of Changbai Mountain & Functional Molecules，Ministry of Education,
Yanbian University, Yanji 133002, China

Received:2010-06-04 Revised:2011-02-11 Online:2011-03-25 Published:2011-03-03
Contact: CUI Sheng-yun E-mail:sycui820@yahoo.com.cn

Abstract

Abstract: The content of reduced glutathione (GSH) in Lycium barbarum L fruits was determined by mass spectroscopy and UV-visible spectroscopy using 5,5＇-dithio-bis-nitrobenzoic acid (DTNB) as the simultaneous derivatizing agent. GSH in Lycium barbarum L fruits was significantly affected by extracellular chemical environment during sample treatment. Extraction with DTNB as the simultaneous derivatizing agent inhibited the transformation or decomposition of GSH due to immediate derivatization during sample treatment. Quantitative determination results indicated that Lycium barbarum L fruits contained GSH in the range of (6.56±0.54) mg/g, which was one of the most GSH-abundant plant species.

Key words: glutathione, 5,5＇-dithio-bis-nitrobenzoic acid (DTNB), Lycium barbarum L, mass spectroscopy, simultaneous derivatization

CLC Number:

O657

ZHU Ya-ling，ZHANG Xiao-yong，CUI Sheng-yun*. Determination of GSH in Lycium barbarum L Fruits by Derivatizing Agent[J]. FOOD SCIENCE, 2011, 32(6 ): 250-255 https://doi.org/10.7506/spkx1002-6630-201106058

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Determination of GSH in Lycium barbarum L Fruits by Derivatizing Agent

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