Effect of Carbohydrates on Activities of Superoxide Dismutase Extracted from Sheep Blood

doi:10.7506/spkx1002-6630-201107023

FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (7 ): 100-103.doi: 10.7506/spkx1002-6630-201107023

• Basic Research • Previous Articles Next Articles

Effect of Carbohydrates on Activities of Superoxide Dismutase Extracted from Sheep Blood

YANG Xue-shan，YANG Xiao-pu，FU Wen-li

College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China

Received:2010-07-28 Revised:2011-02-23 Online:2011-04-15 Published:2011-03-30
Contact: YANG Xue-shan E-mail:yangxs@gsau.edu.cn

Abstract

Abstract: The activity of sheep blood superoxide dismutase (SOD) purified by DEAE Sephadex A-50 column chromatography was determined by pyrogallo autoxidation method after 4, 24, 48, 72 h and 96 h of 25 ℃ incubation in the respective presence of maltose, sucrose, lactose, fucose, glucose, D-fructose, D-trehalose, D-xylose and D-mannose under varying concentration conditions, and the corresponding relative enzyme activities were calculated. Trehalose had a remarkable activating and protecting effect on sheep SOD. High concentrations of maltose and low concentrations of lactose were conducive to stabilizing the enzyme, and other sugars tested had unobvious effect on the enzyme. Therefore, different kinds and amounts of sugar have different effects on sheep SOD.

Key words: superoxide dismutase, carbohydrate, enzyme activity

CLC Number:

Q814

YANG Xue-shan，YANG Xiao-pu，FU Wen-li. Effect of Carbohydrates on Activities of Superoxide Dismutase Extracted from Sheep Blood[J]. FOOD SCIENCE, 2011, 32(7 ): 100-103.

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Effect of Carbohydrates on Activities of Superoxide Dismutase Extracted from Sheep Blood

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