Synthesis and Identification of Two Types of Specific Artificial Antigen for Fenvalerate

doi:10.7506/spkx1002-6630-201107038

FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (7 ): 178-182.doi: 10.7506/spkx1002-6630-201107038

• Bioengineering • Previous Articles Next Articles

Synthesis and Identification of Two Types of Specific Artificial Antigen for Fenvalerate

XIONG Zhen-yu,YANG Yan-yan,LI Pei-wu,ZHANG Qi,ZHANG Wen,DING Xiao-xia

1.Faculty of Life Sciences,Hubei University,Wuhan 430062,China;2.Oil Crops Research Institute,Chinese Academy of Agricultural Sciences,Wuhan 430062,China;3.Key Laboratory of Oil Crops Biology,Ministry of Agriculture,Wuhan 430062,China;4.Quality Inspection and Test Center for Oilseeds and Products,Ministry of Agriculture,Wuhan 430062,China

Received:2010-06-29 Revised:2011-02-25 Online:2011-04-15 Published:2011-03-30

Abstract

Abstract: Fenvalerate, with a typical chemical structure, was selected as the research object in this study. Two types of haptens were synthesized through separately introducing two space arms with different connecting lengths to one end of fenvalerate by organic synthesis. 1H-NMR and LC-MS-MS identifications demonstrated the successful synthesis of haptens, which were designated as Fen 1 and Fen 2. Fen 1 and Fen 2 were separately coupled to bovine serum albumin (BSA), and two kinds of specific immunogens were obtained and named as Fen 1-BSA and Fen 2-BSA. UV-visible spectroscopic analysis indicated that the two artificial immunogens differed significantly from their corresponding heptens and the protein carrier. The coupling ratios between Fen 1 or Fen 2 and BSA were around 9:1 and 10:1, respectively. The results of SDS-PAGE showed that both haptens were conjugated to BSA successfully.

Key words: fenvalerate, hapten, artificial antigen, synthesis

CLC Number:

S481.8

XIONG Zhen-yu,YANG Yan-yan,LI Pei-wu,ZHANG Qi,ZHANG Wen,DING Xiao-xia. Synthesis and Identification of Two Types of Specific Artificial Antigen for Fenvalerate[J]. FOOD SCIENCE, 2011, 32(7 ): 178-182.

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Synthesis and Identification of Two Types of Specific Artificial Antigen for Fenvalerate

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