Detection of Escherichia coli O157:H7 Based on Bio-Bar Codes Probes and Gold Nanoparticles

doi:10.7506/spkx1002-6630-201108040

Abstract

Abstract: A novel assay was developed for the detection of Escherichia coli O157:H7 (E. coli O157:H7) based on bio-bar DNA and gold nanoparticles (AuNPs). The immune reaction between the target and antibodies modified on biofunctionalized magnetic nanoparticles (MNPs) or AuNPs leads to the formation of a sandwich structure of MNPs/target/AuNPs, followed by de-hybridization and release of bio-bar DNA. Due to its ability to hybridize with probe ssDNAs modified on AuNPs, bio-bar DNA could lead to aggregation and color change of AuNPs, which is readily visible with naked eyes, and can be quantified by UV-visible spectroscopy as well. The proposed method had a detection limit of about 102 CFU/mL in both pure broth culture and milk with a detection range of 102－106 CFU/mL. With the aid of bio-bar DNA and AuNPs, detection of E. coli O157: H7 in food is accurate, fast, and easy-operated, which may provide a new tool for food safety monitoring.

Key words: magnetic nanoparticles, gold nanoparticles, bio-bar codes, UV-visible spectroscopy, E. coli O157:H7

CLC Number:

R446.5

PU Xiao-ping，YANG Hai-lin，ZHOU Nan-di. Detection of Escherichia coli O157:H7 Based on Bio-Bar Codes Probes and Gold Nanoparticles[J]. FOOD SCIENCE, 2011, 32(8): 177-181.

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Detection of Escherichia coli O157:H7 Based on Bio-Bar Codes Probes and Gold Nanoparticles

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