Abstract: A three-wavelength UV spectrophotometric method was proposed for the determination of total flavonoid content in Ampelopsis grossedentata. Samples were extracted with ethanol, and the extract was then detected colorimetrically with aluminum chloride as chromogenic agent at the wavelengths of 322, 311 nm and 296 nm. The regression equation of the proposed method was obtained as follows: δA = 0.0171C＋0.0171, which exhibited good linearity in the range of 0 to 40μg/mL, with a correlation coefficient r of 0.9978. The spike recoveries at two spiked levels were between 98.18% and 102.79%, and the precision RSDs (n = 5) were less than or equal to 0.371%. Thus, this method is accurate and precise.

Key words: Ampelopsis grossedentata, flavonoids, three-wavelength UV spectrophotometry