Abstract: Objective: To accelerate and enhance the extraction of polysaccharides from shiitake mushroom by inner ebullition. Methods: A small amount of ethanol was used to wet the raw material for the dissolution of polysaccharides, and a given volume of hot water was then added to boil ethanol entering the raw material, resulting in rapid extraction of polysaccharides. Results: The maximum extraction of polysaccharides was obtained by wetting with 20% ethanol followed by 5 min extraction with a 25-fold volume of 90 ℃ hot water. The extraction yield and purity of polysaccharides obtained after concentration and subsequent ethanol precipitation was 5.05% and 51.6%, respectively. Compared to the traditional decoction method, the optimized method displayed an increase in polysaccharide yield purity respectively by 0.18% and 10.2% and a reduction in extraction time by 85 min. Conclusions: Inner ebullition has distinctive advantages in extracting polysaccharides from shiitake mushroom.

Key words: inner ebullition, polysaccharides, extraction