Screening of High-affinity DNA Aptamers to Salmonella Antigen by SELEX Technique

doi:10.7506/spkx1002-6630-201113042

FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (13): 194-197.doi: 10.7506/spkx1002-6630-201113042

• Bioengineering • Previous Articles Next Articles

Screening of High-affinity DNA Aptamers to Salmonella Antigen by SELEX Technique

LANG Chun-yan1,2，JIANG Shu-xun1,*，SHAO Bi-ying1，CHEN Wen-bing1，FU Bi-zhong1，MIAO Ting-yu1， CHEN Rong-bin3，HUANG Xiao-rong1

1. Fujian Key Laboratory for Technology Research of Inspection and Quarantine, Fujian Entry-Exit Inspection and Quarantine Bureau, Fuzhou 350001, China；2. College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China； 3. Fisheries College of Jimei University, Xiamen 361021, China

Online:2011-07-15 Published:2011-07-02

Abstract

Abstract: Objective: To screen high-affinity aptamers to Salmonella antigen by applying SELEX (system evolution of ligands by exponential enrichment) method and establish a rapid detection method for Salmonella in food. Methods: First, a single stranded DNA (ssDNA) random library with 78 nucleotides in length containing 35 random sequences was constructed. Subsequently, Eupergit C was used as the screening medium in the SELEX method. The binding of aptamers to Salmonella antigen was visualized by Biotin-Streptavidin-Anti-Digoxigenin-AP system to screen high-affinity aptamers out. Results: The binding assay demonstrated a gradual decrease in the absorbance at 405 nm as the number of screening rounds increased. High-affinity aptamers binding to Salmonella antigen were successfully obtained from the initial random ssDNA library. Conclusion: The screening process is simple and can be used for similar targets.

Key words: system evolution of ligands by exponential enrichment (SELEX), Salmonella, antigen, aptamer

CLC Number:

Q789

LANG Chun-yan，JIANG Shu-xun,，SHAO Bi-ying，CHEN Wen-bing，FU Bi-zhong，MIAO Ting-yu，. Screening of High-affinity DNA Aptamers to Salmonella Antigen by SELEX Technique[J]. FOOD SCIENCE, 2011, 32(13): 194-197.

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Screening of High-affinity DNA Aptamers to Salmonella Antigen by SELEX Technique

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