Simultaneous Detection of 3 Amino Acids with Overlapped Fluorescent Spectra by Multi-dimensional Data Analysis

doi:10.7506/spkx1002-6630-201116062

Abstract

Abstract: An orthogonal signal correction-wavelet packet transform-partial least squares (OSC-WPT-PLS) method was proposed for simultaneous detection of tryptophan, tyrosine and phenylalanine with overlapped fluorescent spectra. A program (POSCWPTPLS) was designed to execute relevant calculations and three chemometric methods (OSC-WPT-PLS, WPT-PLS and PLS) were compared in this study. The relative standard errors of prediction (RSEP) were achieved for all components by using OSC-WPT-PLS, WPT-PLS and PLS were 2.80%, 4.35% and 5.14%, respectively. The OSC-WPT-PLS method revealed the best detection efficiency among three methods, which could be successfully applied to determine the contents of tryptophan, tyrosine and phenylalanine in water, Gold-Camel wine and Hetao wine made in Inner Mongolia with a recovery rate of respectively 96.9%－103.2%, 94.1%－105.4% and 95.3%－107.8%.

Key words: multi-dimensional data analysis, fluorespectrophotometry, simultaneous determination, tryptophan, tyrosine, phenylalanine

CLC Number:

O657.3

XUE Yong-lin，REN Shou-xin，ZHANG Yan-bing，GAO Ling. Simultaneous Detection of 3 Amino Acids with Overlapped Fluorescent Spectra by Multi-dimensional Data Analysis[J]. FOOD SCIENCE, 2011, 32(16): 290-294.

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Simultaneous Detection of 3 Amino Acids with Overlapped Fluorescent Spectra by Multi-dimensional Data Analysis

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