FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (17): 109-113.doi: 10.7506/spkx1002-6630-201117021

• Basic Research • Previous Articles     Next Articles

Improvement of Anti-freezing and Fermentation Properties of Frozen Dough by Recombinant Rhizopus chinensis Lipase Combined with Transglutaminase

LI Zhen-ni1,WANG Hong-zi2,HUANG Wei-ning1,*,ZHANG Luan1,JIA Chun-li1,XU Yan3, RAYAS-DUARTE Patricia4   

  1. (1. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; 2. Fuma MiMi (Fujian) Food Industry Co. Ltd., Jinjiang 362216, China; 3. Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China; 4. Food and Agricultural Products Research Center, Oklahoma State University, Stillwater 74078-6055, USA)
  • Published:2011-08-30

Abstract: The effects of Rhizopus chinensis lipase (RCL) and transglutaminase (TG) on anti-freezing and fermentation properties of frozen dough during storage were investigated by using differential scanning calorimetry and F3 rheofermentometer. All doughs were stored at-18 ℃ for 7, 21 days and 35 days. The results showed that glycerol content was decreased and freezable water content was increased with the increase of frozen storage time. RCL and TG could increase significantly glycerol content of frozen dough. The combined application of RCL and TG prevented the formation of ice crystals during frozen storage by lowering freezable water content in dough, and increased viable yeast cell count. Meanwhile, RCL and TG restricted frozen storage to deteriorate dough development, improved yeast fermentation activity, and increased gas retention of frozen dough.

Key words: lipase, transglutaminase, frozen dough, fermentation

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