Isolation and Preliminarily Identification of Cellulase-free Xylanase-producing Strain

doi:10.7506/spkx1002-6630-201117057

Abstract

Abstract: In order to obtain a strain with cellulase-free xylanase activity, a bacterium named as 26T was isolated from paper mill wastewater by substrate screening. The strain 26T was preliminarily identified as Bacillus subtilis by analyzing its physiological and biochemical characteristics and 16S rDNA gene sequence. This strain could grow under the conditions of 50 ℃ and pH 10, and revealed high heat and alkali resistance. The xylanase from it as an extracellular enzyme had an activity of 14.58 U/mL under the optimal fermentation conditions of 37 ℃, 200 r/min and 12 h. Therefore, this strain might have a prosperous application in food industry.

Key words: Bacillus subtilis, xylanase, cellulase, heat resistance, alkali resistance

CLC Number:

Q93.331

CHEN Na，SHENG Ji-ping，SHEN Lin. Isolation and Preliminarily Identification of Cellulase-free Xylanase-producing Strain[J]. FOOD SCIENCE, 2011, 32(17): 273-277.

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Isolation and Preliminarily Identification of Cellulase-free Xylanase-producing Strain

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