Optimization of Hydrolysis Conditions of Xylanase and α-Glucuronidase Mixture for Birch Xylan by  Response Surface Methodology

doi:10.7506/spkx1002-6630-201804019

Abstract

Abstract: Objective: This study aimed to optimize the hydrolysis of birch xylan with a mixture of recombinant thermostable xylanase (XynB) and glucuronidase (AguA) from an engineered strain of Escherichia coli. Methods: The hydrolysis conditions were optimized using combination of one-factor-at-a-time method and response surface methodology. Results: The optimized conditions were obtained as follows: substrate concentration, 4.2 g/100 mL; temperature, 80.66 ℃; pH, 7.65; and XynB/AguA dosage, 60/9 U/g, yielding a reducing sugar concentration of 17.82 mg/mL. Conclusion: The reducing sugar and xylose concentrations were respectively 17.91 mg/mL and 13.66 mg/mL after 4 h of hydrolysis with the enzyme mixture.

Key words: xylanase (XynB), α-glucuronidase (AguA), hydrolysis of xylan, response surface methodology, optimization

CLC Number:

TS201.1

DONG Yuanyuan, LI Yaxian, SHEN Yihong, XUE Yemin. Optimization of Hydrolysis Conditions of Xylanase and α-Glucuronidase Mixture for Birch Xylan by Response Surface Methodology[J]. FOOD SCIENCE, 2018, 39(4): 125-131.

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Optimization of Hydrolysis Conditions of Xylanase and α-Glucuronidase Mixture for Birch Xylan by Response Surface Methodology

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