FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (19): 268-272.doi: 10.7506/spkx1002-6630-201119060

• Nutrition & Hygiene • Previous Articles     Next Articles

Inhibitory Effect of Ethanol Extract from Propolis on Maltase and Sucrase in Rat Intestinal Tract

WANG Guang-xin1,2, DONG Jie2, ZENG Xiao-xiong1, ZHANG Hong-cheng2,*   

  1. (1. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China; 2. Bee Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100093, China)
  • Published:2011-10-12

Abstract: In order to explore the control effect of ethanol extract from propolis (EEP) on postprandial blood glucose, propolis was extracted by ethanol with various concentrations. The contents of total flavonoids and total phenols in EEPs were analyzed by high performance liquid chromatography (HPLC). The inhibitory effect of EEPs on sucrase and maltase in rat intestinal tract was also investigated. The results indicated that EEP extracted by 75% ethanol revealed the highest content of total flavonoids, which was (697.36 ± 6.15) mg rutin/g EEP. However, EEP extracted by 25% ethanol had the highest content of total phenols, which was (386.50 ± 17.40) mg gallic acid/g EEP. Moreover, the EEP extracted by 75% ethanol showed the highest anti-sucrase activity with IC50 of (32.30 ± 0.60) μg/mL. In contrast, the aqueous extract of propolis exhibited the highest anti-maltase activity with IC50 of (32.60 ± 0.20 )μg/mL). Compared with the positive control group, the IC50 of each EEP against sucrase from rat intestinal tract exhibited a significant decrease (P < 0.05).

Key words: propolis, maltase, sucrase, phenolic compound

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