Abstract: In this study, resistant starch was prepared from maize starch with pullulanase. Based on one-factor-at-a-time experiments, the main parameters of pullulanase hydrolysis were investigated by response surface methodology (RSM), including reaction temperature, pH value, reaction time, and pullulanase amount, and a mathematical model describing resistant starch yield was developed. The optimum preparation conditions were achieved as follows: pullulanase amount 12.8 ASPU/g, reaction temperature 46.2 ℃, reaction time 32 h, pH 5.0. Under these conditions, the yield of the maize resistant starch is 46.2%.

Key words: resistant starch, pullulanase, maize starch, response surface methodology (RSM)