Abstract: A high performance liquid chromatography with refractive index detection (HPLC-RI) method was established for the simultaneous quantification of glucose, sucrose and maltose. The chromatographic separation was achieved on a Sugar-D column using a mixture of acetonitrile and water (V/V＝78:22) as mobile phase at a flow rate of 1.0 mL/min. Under these conditions, glucose, sucrose and maltose could be well separated. A good linear relationship for each analyte was observed over the concentration range of 0.50－10.00 mg/mL. The spike recoveries of glucose and sucrose were 94.3%－103.7% and 101.4%－105.1% respectively. The results of uncertainty evaluation indicated that the combined standard uncertainty was 1.92% and the expanded uncertainty was 4.1%. The method was simple, accurate, stable, reproducible, and suitable for the practical determination of glucose, sucrose and maltose in compound saccharated pepsin granules.

Key words: high performance liquid chromatography with refractive index detection (HPLC-RI), compound saccharated pepsin granules, glucose, sucrose, maltose